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Fig. 4 | Radiation Oncology

Fig. 4

From: Development of a high-throughput γ-H2AX assay based on imaging flow cytometry

Fig. 4

Representative images of γ-H2AX foci in human blood lymphocytes irradiated cells with γ-rays (0, 2 and 4 Gy), 1 h after irradiation. Cellular images displayed here show BF, γ-H2AX, γ-H2AX foci mask, DRAQ5 nuclear staining and a composite of γ-H2AX and DRAQ5. The spot counting wizard in the IDEAS® software was used to identify and enumerate γ-H2AX foci in all imagery (40x magnification)

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