Fig. 3

A) Violin plot showing expression of Cav1 across each fibroblast cluster. Note Cav1 expression was observed in all fibroblast clusters. B) Violin plot showing expression of Cav2 in each fibroblast cluster. Cav2 expression was primarily noted in Cluster 2 (green). C) Feature plots showing expression of Cav1 (red, left plot) and Cav2 (green, middle plot) for across all fibroblasts and where overlap expression was appreciated (right plot). D) Representative immunohistochemical staining images at 20x of Cav1 (red) with DAPI counterstain (blue) in irradiated (left image) and non-irradiated (right image) skin. Greater Cav1 staining was appreciated in non-irradiated skin (blue bar; n = 15/group; ****p < 0.0001). E) Representative immunohistochemical staining images at 20x for Cav2 (red) with DAPI counterstain (blue) in irradiated (left image) and non-irradiated (right image) skin. Staining for Cav2 was greater appreciated in irradiated skin (red bar; n = 15/group; ****p < 0.0001). Abbreviations: Cav1, caveolin 1; Cav2, caveolin 2; DAPI, 4’,6-diamidino-2-phenylindole; iR, irradiated; NiR, non-irradiated